Abstract
In this study, we demonstrated selenium (Se) accumulation in Bifidobacterium longum strain (B. longum) and evaluated the effect of Se-enriched B. longum (Se-B. longum) on tumor growth and immune function in tumor-bearing mice. Analysis using high-performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS) revealed that more than 99% of Se in Se-B. longum was organic, the main component of which was selenomethionine (SeMet). In the in vivo experiments, tumor-bearing mice (n=8) were orally administrated with different doses of Se-B. longum alone or combined with cyclophosphamide (CTX). The results showed that the middle and high dose of Se-B. longum significantly inhibited tumor growth. When Se-B. longum and CTX were combined, the antitumor effect was significantly enhanced and the survival time of tumor-bearing mice (n=12) was prolonged. Furthermore, compared with CTX alone, the combination of Se-B. longum and CTX stimulated the activity of natural killer (NK) cells and T lymphocytes, increasing the levels of interleukin-2 (IL-2) and tumor necrosis factor-α (TNF-α), and the leukocyte count of H22 tumor-bearing mice (n=12).
Highlights
Bifidobacterium longum (B. longum) is a lactic acidproducing bacterium that grows naturally in the human gastrointestinal tract and defends the host against viral infection (Otles et al, 2003; Picard et al, 2005)
Some groups used mouse models to study the role of Se in cancer cells and their results showed that Se exhibited antitumor activity in several tumor-bearing mouse models (Chen et al, 2007; Sanmartin et al, 2012)
Selenium accumulation by B. longum B. longum was cultured in medium supplemented with different concentrations of sodium selenite at 38°C for 10 h
Summary
Bifidobacterium longum (B. longum) is a lactic acidproducing bacterium that grows naturally in the human gastrointestinal tract and defends the host against viral infection (Otles et al, 2003; Picard et al, 2005). When Se-B. longum and CTX were combined, the antitumor effect was significantly enhanced and the survival time of tumor-bearing mice (n=12) was prolonged. We used B. longum strain without transporting genes to accumulate Se, determined the Se species in Se-enriched B. longum (Se-B. longum) and investigated the effect of Se-B. longum on solid tumor growth, survival time and immune function of tumor-bearing mice when treated alone or combined with cyclophosphamide (CTX).
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
