Abstract
The activation of a single-chain Fv antibody on the surface of a quartz crystal microbalance (QCM) sensor chip was investigated in order to develop an economical and sensitive immuno-QCM sensor system for use in clinical diagnosis. On the bare gold surface of a QCM sensor chip, approximately 60nm of hydrophilic polystyrene (phi-PS) thin film that specifically binds the polystyrene-binding peptide (PS-tag) was prepared by spin-coating and O(2)-plasma irradiation. When the adsorption of PS-tag-fused anti-ED-B scFv (scFv-PS) onto the phi-PS surface was directly monitored, the maximum density of scFv-PS attained was 1.56μg/cm(2), 1.6-times higher than that of scFv. The specific antigen-binding activity of scFv-PS after solid-phase refolding increased with the density of immobilized scFv-PS, and, consequently, activity 1.7 times higher than that of scFv was retained. The scFv-PS-immobilized QCM sensor chip rapidly allowed the detection of clear signals for antigen at the range of 0.1-10μg/ml, while no signal was detectable for 10μg/ml BSA as a negative control. The scFv-PS-immobilized QCM sensor developed in the present study will therefore be very useful for the rapid and highly sensitive detection of biomarkers, and should be applicable to clinical diagnosis.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
