Abstract
We have recently applied the fluorescent antibody technique for rapid detection of viral antigens in several organs of infected trouts as well as in the supernatant of infected cell cultures (Enzmann, 1978, 1979). For diagnosis of nonacute infections of trout with VHS virus a method was developed which allows the rapid detection of viral antigens in leukocytes after isolation of the white blood cells. Leukocytes have been shown also to become infected during an acute VHS infection and may be functionally impaired during the disease. Fluorescent antibody studies on the persistence of infection and on the detection of antigens in acute infections will be described.
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