Abstract
ObjectiveTo prepare, characterize and evaluate the antioxidant activity of rutin-liposome (RL). MethodsLiposomes of rutin were prepared by film dispersion method and the encapsulation efficiency (EE) was determined by RP-HPLC. Human umbilical vein endothelial cells (HUVECs) were injuried by H2O2 and treated with either free aqueous rutin or the RL delivery systems. The viability of HUVECs was determined by MTT and ELISA. ResultsThe drug delivery system showed uniform rutin loaded nanoparticles with average particle size of (147.20 ± 1.42) nm, polydispersity index of (0.191 ± 0.003) nm, Zeta potential of (−20.0 ± 1.0) mV, and the drug EE was closed to 90.0%. The antioxidant effect of the drug delivery system to H2O2-damaged HUVECs showed that RL could increase injury cells viability compared to free aqueous rutin, which was accompanied with an obvious decrease in malondialdehyde (MDA), lactate dehydrogenase (LDH) while increase the level of nitrogen oxide (NOS). ConclusionThe nanostructured RL is improved on the antioxidant effect and may be treating the different diseases caused by free radicals.
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