Abstract
INTRODUCTIONOne versatile strategy for sample cleanup prior to MALDI-MS analysis uses microscale columns designed for direct sample elution onto the MALDI target plate. This protocol describes the fabrication of a reversed-phase microcolumn designed for this purpose. The microcolumns are prepared from GELoader tips. This protocol has been optimized for sample cleanup prior to MALDI-MS. However, with slight modifications, it works equally well with samples destined for ESI-MS.
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