Preparation of Readily-to-Use Stilbenoids Extract from Morus alba Callus Using a Natural Deep Eutectic Solvent

Jukrapun Komaikul,Tharita Kitisripanya,Supachoke Mangmool,Waraporn Putalun

doi:10.3390/cosmetics8030091

Jukrapun Komaikul, Tharita Kitisripanya + Show 2 more

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https://doi.org/10.3390/cosmetics8030091

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Journal: Cosmetics	Publication Date: Sep 19, 2021
Citations: 8	License type: CC BY 4.0

Affiliation: Mahidol University, Khon Kaen University

Abstract

The consumer and cosmetic industries have recently placed a greater emphasis on ecofriendly solvents for botanical extraction, including natural deep eutectic solvents (NADES). In this study, NADES were prepared for Morus alba callus extraction. The efficiency of extraction from the NADES and methanol was investigated by comparison of the stilbenoids yield and anti-melanogenesis activity. Prior to testing the irritability of a suitable NADES on the reconstructed human epidermis (RhE), the effect of the selected NADES on stilbenoids stability was determined. The results showed that the highest yields of stilbenoids were obtained from choline chloride-glycerol mixtures (Ch1G2) and methanol extracts, with no significant difference in yields (5.06 ± 0.05 and 6.32 ± 0.40 mg/g callus dry weight, respectively). The NADES extracts of M. alba callus showed comparable anti-melanogenesis activity compared to methanol. In term of stability, stilbenoids in Ch1G2 remained stable after six months of storage at 4 °C except resveratrol. Furthermore, Ch1G2 had no irritation effect on RhE. Thus, based on the findings of this study, Ch1G2 is an intriguing green solvent alternative for the extraction of M. alba callus and may be advantageous for the preparation of skin-lightening cosmetics.

Highlights

Extraction is a critical step in the process of obtaining phytochemicals from plant raw materials
The purpose of this study was to determine the capacity of natural deep eutectic solvents (NADES) to extract stilbenoids from M. alba callus and the suitability of a selected NADES for the preparation of the ready-to-use extract for skin lightening products
B16-F10 (CRL-6475), Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS) were purchased from ATCC (Manassas, VA, USA). α-Melanocyte-stimulating hormone (α-MSH), kojic acid, and synthetic melanin were purchased from Sigma-Aldrich

Summary

Introduction

Extraction is a critical step in the process of obtaining phytochemicals from plant raw materials. An organic solvent such as ethanol has been used to extract plant raw materials for cosmetic ingredients. The solvent should be removed or reduced using an efficient system. Organic solvent extracts in cosmetics could cause an unsatisfactory experience for consumers and may occasionally be incompatible with other cosmetic ingredients. Green solvents are gaining popularity as an environmentally friendly and safe alternative that benefits both workers and consumers. Exploring green solvents for the preparation of botanical extracts is a current research trend [1]

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