Abstract
The present work deals with the use of previously tetanus immunized horses for preparation of rabiesantiserum in addition to investigate the effect of simultaneous immunization of horses against rabiesand tetanus in order to prepare a bivalent anti-rabies and tetanus serum. The obtained results of appliedserological tests revealed that the presence of tetanus antibodies in previously immunized horses did notaffect the levels of induced rabies antibodies while simultaneous immunization resulted in high titers ofrabies antibodies (256 IU/ml) as compared to such titers resulted in horses immunized with rabiesvaccine alone (128 IU/ml). These findings suggest an enhancing action of tetanus antigen on antibodyresponse against rabies and the prepared anti-serum can be used in emergency bite injuries in animalsprotecting them against both of rabies and tetanus infections as noticed in experimentally rabies infectedmice.
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