Abstract
Neem protein isolate (NPI) was prepared from neem seed cake (NSC) resulting in five fold increase of crude protein content, amounting to 82%. The recovery of protein was highest (49%) when the NSC was solubilised at pH 12 for 2h and precipitated at pH 4. Crude fibre, nitrogen free extract and total ash contents were significantly (P<0.05) reduced substantially in the isolate compared to the NSC. The NPI has a balanced amino acid composition with high levels of methionine and arginine. A significant decrease of anti-nutritional factors such as oxalate (87.50%), phytic acid (70.50%), tannin (72.73%), cyanide (50.98%) and azadirachtin content (79.06%) was found in the isolate, implying that the isolation process was effective in the removal of toxic components. A significant (P<0.05) increase in the in vitro protein digestibility (90.4) was also obtained for the protein isolate, suggesting that the NPI as a promising feed ingredient.
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