Preparation of porous magnetic molecularly imprinted polymers for fast and specifically extracting trace norfloxacin residue in pork liver.

Abstract

Here, magnetic molecularly imprinted polymers were designed for norfloxacin via oil-in-water emulsifier-free emulsion method. It was prepared by simply mixing norfloxacin, methacrylic acid-co-ethylene glycol dimethacrylate copolymer, and Fe3 O4 together at room temperature. Characterized by multiple analytical tools, the particle size, pore size, pore volume, specific surface area, and saturation magnetization of the product were about 30µm, 10-500nm, 2.92mL/g, 105.84 m2 /g, and 3.052 emu/g, respectively. And the adsorption capacity was high at 27.04mg/g towards norfloxacin. Combined with ultra high performance liquid chromatography, it was used to determine norfloxacin in real samples. Average recoveries were above 77.44% with relative standard deviations between 1.21 and 6.85% at three spiked levels (n=3) for lake water and pork liver. The determination was achieved for the most complex biosample pork liver spiked with norfloxacin low to 30ng/g, about 100 times less than the maximum residue limit regulated by Commission of the European Communities. All evidences demonstrated that the magnetic molecularly imprinted polymers can be used in practice for monitoring norfloxacin either in environmental water or meat product with high accuracy and reliability.