Preparation of Porcine Allogenic Acellular Diaphragm Matrix and its Application for Umbilical Hernioplasty in Pig

Abstract

Background: The surgical reconstruction of large abdominal defects with application of surgical mesh of synthetic origin resulted a number of potential clinical complications; although it is having good tensile strength. Acellular bio-mesh derived from slaughtered animals could be better alternative for surgical management abdominal wall defects. The present study aimed to evaluate bio-detergent based decellularized allogenic porcine acellular diaphragm matrix in umbilical hernioplasty in pig. Methods: For the present study sodium deoxycholate was used as bio-detergent for preparation of porcine allogenic acellular diaphragm matrix (ADM). All total eighteen (18) numbers of piglets aged 2-3 months, body weight 8-14 kg and either sex with umbilical hernia equally divided in to three groups. Group A was subjected to application of ADM; while in Group B injection of methylprednisolone along with ADM application and Autologous Tunica Vaginalis was used for hernioplasty in Group C. Various parameters viz clinical, gross evaluation of surgical wound, haemato-biochemical and indirect ELISA of blood and serum was carried out as per standard methods. Result: Decellularization protocol carried out with sodium deoxycholate as bio-detergent@ 2% concentration resulted complete decellularization of porcine diaphragm and preserved the distinctive, natural, three-dimensional collagen structure within the prepared matrix. Clinical and haemato-biochemical observations following umbilical hernioplasty in pig with ADM promises its clinical utility. The immune response of host tissue to bio-mesh as revealed by Indirect ELISA was negligible.