Abstract
The extraction of Ramulus mori polysaccharides (RMPs) was optimized using response surface methodology (RSM). The optimal process conditions, which gave the highest yield of RMPs (6.25%) were 80 °C, 50 min, and a solid–liquid ratio of 1:40 (g/mL), with the extraction performed twice. The RMPs contained seven monosaccharides, namely, mannose, rhamnose; glucuronic acid, glucose, xylose, galactose, and arabinose, in a 1.36:2.68:0.46:328.17:1.53:21.80:6.16 molar ratio. The glass transition and melting temperatures of RMPs were 83 and 473 °C, respectively. RMPs were α-polysaccharides and had surfaces that resembled a porous sponge, as observed by scanning electron microscopy. RMPs inhibited the proliferation of Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa and showed antioxidant activity (assessed by three different methods), although it was generally weaker than that of vitamin C. RMPs showed anti-inflammatory activity in a concentration-dependent manner. This study provides a basis for exploring the potential uses of RMPs.
Highlights
Mulberry (Morus alba L.) is a perennial woody plant with many ecological effects, including improving air quality, protecting of water resources, soil integration, and improving of microclimates [1].In recent years, mulberry has been used to restore vegetation in the Three Gorges Reservoir Region, control rocky desertification, and reconstruct ecological landscapes in China [2,3]
The results showed that treatment with Ramulus mori polysaccharides (RMPs) at concentrations of 0.5, 1.0, 2.5, and 5.0 mg/mL had no obvious toxic effect on cell growth compared with the control group
R. mori was obtained from the mulberry breeding center at Southwest University, Chongqing, China
Summary
Mulberry (Morus alba L.) is a perennial woody plant with many ecological effects, including improving air quality, protecting of water resources, soil integration, and improving of microclimates [1]. Mulberry has been used to restore vegetation in the Three Gorges Reservoir Region, control rocky desertification, and reconstruct ecological landscapes in China [2,3]. Mulberry leaves are used to rear the domesticated silkworm, with constant pruning critical for the accumulation of leaves to feed more silkworms. Tons of mulberry branches are discarded and burned, representing a significant waste of resources and causing substantial environmental pollution. Ramulus mori, is a traditional Chinese medicine.
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