Abstract
Millimeter-sized polydopamine (PDA) modified three-dimensional interconnected macroporous silica (PDA/3DIMS) with high porosity, large pore volume and pore diameter was prepared and used as the support to immobilize commercial laccase (Lac) from Denilite II S. The skeleton of 3DIMS is constructed by silica nanofilm, so the PDA/3DIMS is constructed by PDA/SiO2/PDA sandwich nano-film. The immobilization conditions, incubation time, pH and enzyme concentration were optimized, and the properties of the immobilized laccase were investigated. The enzyme activity of Lac-PDA/3DIMS (295.9 U/g) was higher than that of Lac-3DIMS (222.2 U/g). The stability and reusability of the two immobilized laccases were both improved comparing with the free laccase. Lac-PDA/3DIMS exhibited higher retained activity (73.6%) than Lac-3DIMS (31.8%) after reused for ten times. Lac-PDA/3DIMS retained more than 84.0% initial activity after storage for a month, however, the free laccase and Lac-3DIMS were 7.1% and 48.6%, separately. These advantages revealed that PDA thin layer plays an important role to improve the enzymatic activity of immobilized laccase, and the PDA/3DIMS material is a very promising support for enzyme immobilization.
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