Abstract
ABSTRACTA novel method to prepare capillary coatings using dopamine (DA) and hyaluronic acid (HA) was mentioned in this paper. Firstly, dopamine was oxidized to form polydopamine and attached to the inner wall of the capillary spontaneously. HA attached to the polydopamine by electrostatic interaction to form a polydopamine-hyaluronic acid (PDA-HA) coating. Protein adsorption on the inner surface of capillary was suppressed, and the proteins of lysozyme (Lys), cytochrome c (Cyt-c), bovine serum albumin (BSA) and ribonuclease A (RNase A) were baseline separated obviously using capillary electrophoresis (CE) within 20 minutes.
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