Preparation of poly(caffeic acid)-CoP nanoparticle film on electrode surface and sensitive voltammetric detection of acetaminophen

Abstract

Acetaminophen (APAP) is regarded as one of the most commonly used analgesic and antipyretic drugs in the word due to its antioxidant and anti-inflammatory properties. In recent years, APAP electrochemical sensors have achieved rapid development and some novel nanomaterials or nanocomposites have been employed in the construction of these sensors. In the present work, poly(caffeic acid)-CoP nanoparticle film, PCA-CoP, was prepared on a glassy carbon electrode (GCE) surface based on a two-step electrochemical method. APAP exhibited the remarkable electrochemical response on GCE/PCA-CoP and the sensitive determination of APAP was realized using differential pulse voltammetry under optimal experimental conditions. A high accuracy, good selectivity, excellent reproducibility and satisfactory stability were involved in the proposed electrochemical sensor. H2O2 induced the acute oxidative injury of human umbilical vein endothelial cells, HUVECs, within 1 h. The measurement of APAP in the growth medium that was used for the 12-hour cell culture and the cellular morphology images revealed the uptake of this drug by the oxidation-injured cells and the cellular activity recovery in the presence of APAP. It suggests a protective effect of APAP at low dose against the oxidative injury of HUVECs.