Abstract

Dopamine (DA) and l-tyrosine (l-Tyr) are neurotransmitters involved in various neuropsychiatric disorders. Therefore, it is important to monitor their levels for diagnosis and treatment. In this study, we synthesized poly (methacrylic acid)/graphene oxide aerogels (p(MAA)/GOA) by in situ polymerization and freeze-drying using graphene oxide and methacrylic acid as substrates. Then, the p(MAA)/GOA were applied as solid-phase extraction adsorbents to extract DA and l-Tyr from urine samples, followed by quantification using high performance liquid chromatography (HPLC). The p(MAA)/GOA showed better adsorption performance for DA and l-Tyr than commercial adsorbents, likely as a result of the strong adsorption of the target analytes via π–π and hydrogen bonding interactions. Further, the developed method had good linearity (r > 0.9990) at concentrations of DA and l-Tyr of 0.075–2.0 and 0.75–20.0 μg mL−1, respectively, as well as a limit of detection of 0.018–0.048 μg mL−1, limit of quantitation of 0.059–0.161 μg mL−1, spiked recovery of 91.1–104.0%, and interday precision of 3.58–7.30%.The method was successfully applied to determine DA and l-Tyr in the urine samples of patients suffering from depression, demonstrating its potential for clinical applications.

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