Abstract
A novel photoluminescent glucose nanosensor was facilely prepared by coupling glucose oxidase (GOx) with poly-l-lysine coated oxygen nanosensors via a glutaraldehyde-mediated Schiff-base reaction. The GOx molecules residing on particle surface catalyzed glucose with the expense of oxygen, which was detected by the sensing particle core incorporated with the reference dye coumarin 6 and oxygen probe Pt(II)-meso-tetra(pentafluorophenyl)porphine. The proposed glucose nanosensors (∼150nm in hydrodynamic diameter) had a quick response time varied from less than 2min to 4min. Glucose calibration was performed with ratiometric photoluminescence and time-resolved fluorescence (TRF) respectively, and a series of calibration plots were constructed according to determination time. In comparison, the ratiometric method resulted in wide dynamic range (e.g. 2–10mM) and high limit of detection (∼1–2mM), while the TRF mode gave narrow dynamic range (e.g. 1–6mM) with low detection limit (∼0.1–0.2mM). Finally the enzymatic glucose nanosensors were tested in human serum samples with a TRF microplate reader.
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