Abstract
Permeation of insulin through grafted PTFE films was controlled by pH decline which was caused by the enzymatic reaction of glucose oxidase (GOD) with glucose. The PTFE-g-P(AAc-co-AAm) films were prepared by the combined use of oxygen plasma pretreatment and photografting. The immobilization of GOD was performed by condensation with grafted polymer chains. The maximum amount (5 mmol/g) of GOD immobilized to P(AAc-co-AAm) was obtained at around 4 hours of the reaction time. The permeation of insulin through the GOD immobilized ePTFE-g-P(AAc-co-AAm) films can be enhanced through the addition of glucose, and then further Catalase. The enzymatic activity of immobilized GOD decreased to 25% of native GOD. However, the enzymatic activity holds for prolonged preservation, e.g., more than 30 days significantly.
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