Abstract

We report the preparation of photodynamic materials from electrospun nanofibers of P(MMA-co-MAA) co-polymer that were doped with montmorillonite (MMT), and further functionalized with the cationic photosensitizer methylene blue (MB). The resultant composite nanofibers were characterized by spectroscopic (infrared, UV − vis, fluorescence) and physical (SEM/TEM, gravimetric) methods. Addition of MMT (0–6 wt%) in the electrospinning process increased the adsorption of the methylene blue photosensitizer from 21.5 mg (67.1 μmol)/g P(MMA-co-MAA) parent copolymer to 30.0 mg (93.8 μmol)/g P(MMA-co-MAA)/MMT-6 composite nanofibers. The MB-decorated P(MMA-co-MAA)/MMT-6 composite nanofibers showed a 99.997% (4.8 log units) and 97% (1.8 log units) reduction in CFU/mL against Staphylococcus aureus (ATCC-6538) and Escherichia coli strain 8099, respectively, after visible light illumination (LED lamp, 30 min, 35 ± 5 mW/cm2), demonstrating that the increased photosensitizer loading attributable to MMT doping led to a 1–2 log unit increase in photodynamic inactivation efficacy over the non-doped MB-decorated P(MMA-co-MAA) parent copolymer (99.9% and 84% reduction for S. aureus and E. coli, respectively). The results demonstrate that MMT and similar additives may be a universal method for increasing the adsorption capacity of cationic photosensitizers in electrospun nanofibers, thereby generating materials with improved photodynamic inactivation efficacy without significantly changing the fiber morphology, cost, or ease of production.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.