Abstract
Selenium is a toxic inorganic form at very low concentration in life, while the organic-selenium compounds are appreciable interest and various of them have essential roles in nutritional science and cell biochemistry. Selenium-enriched yeast (Se-yeast) is a public form of selenium used to additional dietary intake of this essential trace mineral. In this study, an organic selenocystine by using locally isolated bakery yeast (Saccharomyces cerevisiae)is prepared. A novel locally prepared date extract media enriched by 0.2% potassium phosphate (KH2PO4), 0.6% ammonium sulfate is adopted as alternative culture media. Selenium salt is used in different concentrations (30, 60, 120 and 240 μg/mL), which are added to the yeast culture media. While the best concentration of selenium added is 30μg/mL, it achieves optimal conditions for the growth of red color yeast identical to the standard. The organic selenocystine is analyzed by High Performance Liquid Chromatography (HPLC) and Atomic Absorption Spectrophotometer (AAS) as compared with standard product obtained from Sigma. Results confirmed the formation of similar selenocystine products. .
Highlights
Selenium is a trace element, with atomic weight of 79 and is within the group in the periodic table of elements [1]
It is necessary to take consideration of the situation to avoid discouraging the growth of yeast, which can occur because of inorganic selenium salt concentration [28, 29], in addition to the quality and quantity of organic selenocystine as an output
The inorganic selenium was added with each concentration used in the experiment in form of six doses and interval with one hour to avoid the affection of the yeast growth
Summary
Selenium is a trace element, with atomic weight of 79 and is within the group in the periodic table of elements [1]. Since the selenium linked to the amino acid Cystine that confirms the importance of nutritional yeast since 1910 [23], and yeast contains 70 trace elements with organic forms [24]. The study aimed to produce organic selenocystine using locally bread yeast isolation and locally prepared liquid media from date's juice as alternative culture media.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
