Abstract
Poly(D,L-lactic-co-glycolic acid) has been extensively used as a controlled release carrier for drug delivery due to its good biocompatibility, biodegradability, and mechanical strength. In this study, porous PLGA microspheres were fabricated by an emulsion-solvent evaporation technique using poly ethylene glycol (PEG) as an extractable porogen and loaded with protein (lysozyme) by suspending them in protein solution. For controlled release of protein, porous microspheres containing lysozyme were treated with water-miscible solvents in aqueous phase for production of pore-closed microspheres. The surface morphology of microspheres were investigated using scanning electron microscopy (SEM) for confirmation of its porous microstructure structure. Protein property after release was observed by enzymatic activity assay. The pore-closing process resulted in nonporous microspheres which exhibited sustained release patterns over an extended period.
Highlights
Biodegradable polymeric microspheres as an injectable depots for proteins or peptides have been extensively investigated for the past decades [1,2,3,4,5,6,7]
poly ethylene glycol (PEG) was selected as the water extractable porogen to make the porous PLGA microspheres [17]
For preparing the porous microspheres, a blend mixture of PEG and PLGA was co-dissolved in a disperse phase of methylene chloride (MC), and the volatile solvent was slowly removed from the embryonic MC droplets within the aqueous continuous phase
Summary
Biodegradable polymeric microspheres as an injectable depots for proteins or peptides have been extensively investigated for the past decades [1,2,3,4,5,6,7]. The porous PLGA microspheres were fabricated by an emulsion solvent evaporation technique using PEG as an extractable porogen and loaded with protein by suspending them protein solution, and their pores were closed by treating with water-miscible solvents. Pore-closing of lysozyme loaded porous microspheres in aqueous condition: Blank porous PLGA microspheres were dispersed in deionized water by vortexing.
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