Abstract
An electrode for uric acid has been prepared by using an iodide selective electrode with the uricase enzyme. The iodide selective electrode used was prepared from 10% TDMAI and PVC according to our previous study. The enzyme was immobilized on the iodide electrode by holding it at pH 7 phosphate buffer for 20 min at room temperature. The H2O2 formed from the reaction of uric acid was determined from the decrease of iodide concentration that was present in the reaction cell. The potential change was linear in the 2 × 10-5 to 2 × 10-4 M uric acid concentration (3 - 34 mg uric acid/100ml blood) range. Uric acid contents of some blood samples were determined with the new electrode and consistency was obtained with a colorimetric method. The effects of pH, iodide concentration, the amount of enzyme immobilized and the operating temperature were studied. No interference of ascorbic acid, glucose and urea was observed.
Highlights
Enzyme electrodes are being used for the measurement of different organic substrates
The quantum dots (QDs) were used as an indicator to reveal fluorescence property of the system resulting from enzymatic reaction of uricase and HRP, which is involved in oxidizing uric acid to allaintoin and hydrogen peroxide
Uric acid is oxidized by air oxygen in the presence of uricase enzyme and hydrogen peroxide is formed, which reacts with iodide ion quantitatively
Summary
Enzyme electrodes are being used for the measurement of different organic substrates. In some of these procedures the enzyme uricase is used This enzyme catalyzes the oxidation of uric acid to allontoin in the presence of carbon dioxide and hydrogen peroxide is formed. The QDs were used as an indicator to reveal fluorescence property of the system resulting from enzymatic reaction of uricase and HRP (horseradish peroxidase), which is involved in oxidizing uric acid to allaintoin and hydrogen peroxide. Uricase was trapped in plasticized PVC and iodide ion selective electrode was used to monitor iodide This electrode has an average slope of 63 mV/ten uric acid. This study describes the preparation and application of a new potentiometric uric acid sensor
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