Preparation of new molecularly imprinted quartz crystal microbalance hybride sensor system for 8-hydroxy-2′-deoxyguanosine determination

Abstract

The routine measurement of 8-hydroxy-2′-deoxyguanosine (8-OHdG) in biological samples is a difficult analytical problem due to the low levels of the analyte and complex matrix. A new 8-OHdG imprinted quartz crystal microbalance (QCM) sensor has been developed for selective determination of 8-OHdG in serum samples. To fulfil the desired results, we have used methacryloyl aminoantipyrine-Fe(III) [MAAP-Fe(III)] and methacryloyl histidine-Pt(II) [MAH-Pt(II)] as metal-chelating monomers via double metal coordination–chelation interactions for the preparation of additional selective molecular imprinted polymers (MIP). The study includes the measurement of binding interaction of 8-OHdG imprinted quartz crystal microbalance (QCM) sensor, selectivity experiments and analytical performance of QCM chip. The obtained results have showed that the application of double metal–chelate monomer systems has been more effective than single metal–chelate monomer systems. In this study, the detection limit and the linear working range were found to be 0.0075 and 0.0100–3.5 μM, respectively. The affinity constant ( K affinity) was found to be 1.54 × 10 5 M −1 for 8-OHdG using MAH-Pt-8-OHdG–MAAP-Fe based thin film. Also, selectivity of prepared QCM sensor was found as being very high in the presence of competitive species. At the last step of this procedure, 8-OHdG level in blood serum which belongs to a intestinal cancer patient was determined by the prepared QCM sensor.