Preparation of new hybrid organic/inorganic polymeric chiral stationary phases for ligand-exchange chromatography

Abstract

Three new hybrid organic/inorganic polymeric ligand-exchange chiral stationary phases were developed by radical chain transfer reaction and surface grafting on silica gel, and successfully used for the enantioseparations of dl-amino acids and dl-hydroxyl acids. The resolutions were achieved by using water containing 2.0 × 10 −4 mol/L of CuAc 2 as a mobile phase, column temperature of 40 °C, flow rate of 1.0 mL/min and detection at UV 254 nm. The elution order of d-isomer before l-isomer was observed for all dl-amino acids resolved except dl-Pro.