Preparation of Nanographene and Its Effect on the Correlation Between N-Terminal Pro Brain-Type Natriuretic Peptide, Peripheral Blood Copeptin Level and Cardiac Function Grading of Patients with Heart Failure

Abstract

At present, biosensors based on nanomaterials are increasingly adopted in clinical detection of heart failure (HF) patients. This work aimed to analyze the correlation between N-terminal pro brain-type natriuretic peptide (NT-proBNP) and copeptin (CPT) levels in order to study the grading of heart function in patients with HF under the fluorescence platform built on nanographene. The and detection principle of of nanographene solution was prepared, and After its physical and chemical properties were tested, the nanographene solution was then used to detect the expression levels of NT-proBNP and CPT in patients with heart failure. Seventy-two patients with heart failure who came to hospital were selected as the observation group, and another 50 healthy people with physical examination were selected as the control group. The results showed that the constructed nanographene solution mixed with NT-proBNP didn’t exhibit fluorescence quenching. Therefore, it was feasible to detect NT-proBNP with nanographene solution. The test results obtained after the detection of different concentrations of nanographene showed that 20 μg/mL nanographene had the best fluorescence quenching effect. The ROC curve of the constructed nanographene had improved specificity in judging heart failure and had better diagnostic performance. The levels of pro-brain natriuretic peptide and copeptin in patients with heart failure were obviously higher than those in the observation group, and there was considerable difference between the two (P < 0.05). After correlation analysis, it was found that the levels of terminal pro-brain natriuretic peptide and copeptin in patients with heart failure were positively correlated with the grade of heart function in patients with heart failure. In summary, the nanographene solution used in this study can accurately detect the expression level of NT-proBNP in patients with heart failure, and the accuracy and efficiency of diagnosis were improved in patients with heart failure. Moreover, the higher the grade of cardiac function in HF patients, the higher NT-proBNP and CPT levels, and there was positive correlation between cardiac function grade and NT-proBNP and CPT levels.