Preparation of nano hydroxyapatite loaded with syrian inula extract against dental caries

Abstract

Dental caries and oral infections result from various causes, and the germs responsible for these diseases show an increase in resistance towards the standard antibiotics used in treatment due to the inappropriate and indiscriminate use of these antibiotics. Research has directed towards making use of natural products to treat these infections. In this paper, a study was conducted to prepare nanoparticles of hydroxyapatite based on pure chemical materials. The prepared particles were characterized using XRD, FTIR, and AFM techniques, tests confirmed the formation of hydroxyapatite nanoparticles 80 nm. Active compounds were extracted from the Syrian Inula plant using an ethanol solvent. The active compounds present in the extract were identified using gas chromatography technology. Equipped with a GC-MASS mass spectrometer. The plant extract was loaded onto the prepared hydroxyapatite particles. The main bacterial species causing dental caries were obtained from Aleppo University laboratories. The sensitivity of the tested germs to a group of standard antibiotics and the prepared hydroxyapatite nanoparticles modified with the plant extract were studied. The results proved the effectiveness of the prepared particles against the studied germs. It exceeded the effectiveness of all studied antibiotics.