Preparation of mussel-inspired 3D polycaprolactone scaffolds and grafted with rhBMP-2 for bone tissue engineering

Abstract

Event Abstract Back to Event Preparation of mussel-inspired 3D polycaprolactone scaffolds and grafted with rhBMP-2 for bone tissue engineering Sang Jin Lee1, 2, Wan-Kyu Ko1, Na Re Ko1, Min Heo1, Su A Park2 and Il Keun Kwon1 1 Kyung Hee University, Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, Korea 2 Korea Institute of Machinery and Materials, Department of Nature-Inspired Nanoconvergence Systems, Korea Introduction: In the tissue engineering area, the bio-compatible scaffold for bone tissue regeneration should provide not only surface of excellent hydrophilicity for cell attachment onto substrate, but also binding an efficiency of bio-active molecule such as growth factor to promote a differentiation of cells. In this work, thus, we prepared a recombinant human bone morphogenic protein-2 (rhBMP2) grafting on three-dimensional (3D) polycaprolactone scaffold (PCLS)[1],[2] using polydopamine (DOPA) chemistry. Material and Method: The DOPA coated PCL scaffold was characterized by contact angle, water uptake, and x-ray photoelectron spectroscopy (XPS) test to certify whether coated DOPA or not. In order to systematize the loading efficiency of rhBMP2, we examined the releasing test for 21 days. In vitro cell study, pre-osteoblast like MC3T3-E1 cells were seeded on PCL scaffold (PCLS), DOPA coated PCL scaffold (PCLSD), and varying concentration of rhBMP2 grafted onto scaffold PCLSD 100, PCLSD 500 (100ng/ml, 500ng/ml loaded), respectively. They were analyzed by cell proliferation, alkaline phosphatase activity, and real time polymerase chain reaction in order to verify an osteogenic activity of cells. Results and Discussion: In results, we demonstrated that our fabricated a hybrid scaffold well coated with DOPA, and enable both only high loading efficiency and sustained release of rhBMP2. In the in vitro results, the developed 3D porous scaffold promoted the pre-osteoblast attachment, cell proliferation. Moreover they encouraged an osteoconductivity compared to rhBMP2-free scaffold. Conclusion: All of results indicated that our manufactured 3D porous scaffold would be useful and potential application for bone tissue engineering field.