Preparation of mineralized collagen-polyose based biomimetic scaffold material for bone tissue Engineering

Abstract

Objective To prepare a novel bioactive and degradable scaffold with mineralized collagenpolyose based composite by biomimetic synthesis for bone tissue engineering and explore the compatibility of osteoblast culturing on the scaffold.Methods Using the cross-linking product of collagenⅠ and sodium hyaluronate as the template,the calcium phosphate was deposited on it to produce a mineralized composite.The 3-D porous scaffolds were prepared by liquid phase separation after the mineralized composite combining with polylactic acid (PLA) and NaCl.The materials and scaffolds were investigated by x-ray diffraction (XRD),scanning electronic microscopy (SEM) and universal testing machine.In addition,inverted microscope,fluorescence microscope,SEM,Cell Counting Kit-8 assay and alkaline phosphatase (ALP) assay were introduced to analyze the growth,function and compatibility(morphology,proliferation and differentiation ) of osteoblast-like cell on the scaffolds.Results The degree of hydroxyapatite (HA) crystals in the composite was low and the size was tiny,which were similar to that of nature bone.The SEM micrographs showed that the scaffolds possessed 82% of porosity and the pore size was about 200 μm to 650 μm.Cells on the scaffolds spread well and presented a high proliferation rate and differentiation level.Conclusion The novel scaffolds are simiar to nature cancellous (spongy) bone both on structure and in property and might be used as one of the optimal scaffolds material for bone tissue engineering. Key words: Mineralized collagen-polyose based composite; Nano-hydroxyapatite; Bone tissue engineering; Scaffold; Cross-linking