Preparation of milk fat globule membrane ingredients enriched in polar lipids: Composition characterization and digestive properties

Abstract

In this study, milk fat globule membrane (MFGM) ingredients enriched in polar lipids was prepared using membrane filtration, including microfiltration, diafiltration, and ultrafiltration from butter serum powder. Polar lipids (phospholipids, sterols, and gangliosides) in prepared MFGM ingredients were analyzed by 31P NMR, GC-MS, and UPLC-MS/MS, respectively. The lipolysis degree and microstructure of MFGM ingredient and soybean lecithin-emulsification during in vitro digestion were also analyzed. Microfiltration showed higher concentration efficiency than ultrafiltration which increased 2.16% and 2.73% in phospholipid, respectively. Moreover, diafiltration concentrated more polar lipids (6.39% of phospholipid) than microfiltration. MFGM ingredients had high level of sphingomyelin (1.27-1.36%) and ration of GD3 than GM3 of 9.25-9.88-fold of total gangliosides. The different lipolysis behavior between MFGM ingredient-emulsification and soybean lecithin-emulsification were correlated with their different polar lipid compositions. Phospholipids from both MFGM ingredients and soybean lecithin could help maintain the initial structure during the gastric digestion. These results could provide a scientific basic for developing high-polar lipids food, particularly infant formulas and special functional foods.