Abstract
In this investigation micron-sized monodisperse magnetic composite polymer particles with amino and amide functional groups were prepared considering their applications in biotechnology. First, polystyrene/poly (acrylic acid-acrylam- ide-N-N-methylene-bis-acrylamide) [PS/P(AA-AAm-MBAAm)] composite polymer particles were prepared by seeded copolymerization. The carboxyl groups present on or near the particles surface were modified by amine-nucleophile, ethylene diamine (EDA), through pre-activation with dicyclohexyl carbodiimide as coupling agent. The aminated particles were then magnetically modified and named as aminated-Fe3O4 composite particles. Formation of such magnetic composite particles was confirmed by scanning electron micrographs, FTIR-spectra and magnetic susceptibility measurement. The produced composite particles were paramagnetic. To see the relative hydrophilic character of the particles surface the adsorption behavior of trypsin (TR) as biomolecule was studied on PS particles and aminated-Fe3O4 composite particles. The magnitude of adsorbed TR on PS particles was higher than that on aminated-Fe3O4 composite particles.
Highlights
In recent years polymer particles with well defined particle size and various reactive functional groups on the surface are attracting much interest because of their applications in a broad range of fields, e.g. as binders in paints, adhesives, paper coating, textile and as a solid support in the biochemical and biological field as well as in catalysis and calibration standards [1,2,3,4,5]
Polystyrene/poly [PS/P(AA-AAm-MBAAm)] composite polymer particles were prepared by seeded copolymerization
The carboxyl groups present on or near the particles surface were modified by amine-nucleophile, ethylene diamine (EDA), through pre-activation with dicyclohexyl carbodiimide as coupling agent
Summary
In recent years polymer particles with well defined particle size and various reactive functional groups on the surface are attracting much interest because of their applications in a broad range of fields, e.g. as binders in paints, adhesives, paper coating, textile and as a solid support in the biochemical and biological field as well as in catalysis and calibration standards [1,2,3,4,5]. Dispersion polymerization in non-aqueous media allows successful preparation of micron-sized polymer particles with ease [28,29,30,31,32,33] This methodology has suffered drawbacks when functional monomers were employed [34, 35]. The present work aims to describe the preparation of monodispersed micron-sized magnetic composite particles bearing amino and amide groups on or near the particles surface. The produced di-functional magnetic latexes, named as aminated-Fe3O4 latexes, would offer advantagees in biomedical applications as amino functional group can be utilized for coupling of affinity ligands or binding of biomolecules and the remaining amide functional group can simultaneously be used to modify the particles surface or even can act as spacer among the immobilized biomolecules preventing self reaction
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