Preparation of microencapsulated human embryo adrenal cortical cells and their morphology and functions

Abstract

Objective To study the preparation of microencapsulated human embryo adrenal cortical cells and their morphology and functions. Methods Single cell from human embryo adrenal cortex was made, enwrapped with better biological consistent alginate poly-L-lysine alginate (APA) and microencapsulated adrenal cortical cells were developed. The isolated adrenal cortical cells (group A) and microencapsulated adrenal cortical cells (group B) were cultured separately. The cortisol levels in the culture fluid at 22 nd, 46 th, 70 th h were determined in the two groups, respectively. After ACTH (125 ng/L) addition into the culture fluid for stimulating culture for 2 h, the cortisol levels in the culture fluid at 24 th, 48 th and 72 nd h were measured again. Results Morphologically, the total cell survival rate of the embryonic adrenal cells was 92% by using mechanical methods, while the survival of microencapsulated cells was 83% . Microencapsulated adrenal cells shape was intact, evenly distributing in APA. The surface of mi-croencaps was smooth. The cortisol levels in two groups had no significant difference. Microencapsulation had no obvious influence on adrenal cell activity. Conclusion The microencapsulation had no harmful to the survival and secretion function of adrenal cells. Key words: Adrenal cortex; Cell,cultured; Adrenal cortex hormones; Biocompatible materials