Abstract
Magnetic molecularly imprinted polymer (MMIP) was successfully synthesized with acrylamide as a functional monomer and clenbuterol (CLB) as a template molecule. The synthesized MMIPs were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier transform infrared spectrometry (FT-IR). MMIPs were used to identify and bind CLB as a solid phase extraction material. The experiment data were fitted by the Freundlich isotherm adsorption model. The results show that MMIPs have excellent recognition performance for CLB. MMIPs were successfully applied as adsorbents to preconcentrated CLB in pork samples and detected by HPLC with UV. The limit of detection (LOD) and limit of quantification (LOQ) were 4.27 μg/L and 14.2 μg/L, respectively. The spiked recovery rates ranged from 94.44% to 102.29%. Therefore, the prepared MMIPs can be used for selective preconcentration of CLB content in complex animal-derived food samples.
Highlights
Clenbuterol (CLB) is one of the most common β-adrenergic agonists that can be used in the treatment of diseases such as bronchial asthma and chronic bronchitis as a bronchodilator agent in the clinic treatment [1,2,3]
A large amount of CLB can be used as feed additives to significantly promote the growth of the animal and increase the lean rate
The prepared Fe3O4 nanoparticles are prone to magnetic flocculation, so that the result is that nanoparticles lack monodispersity. erefore, the surface of Fe3O4 particles was modified with PEG to avoid electrostatic agglomeration
Summary
Clenbuterol (CLB) is one of the most common β-adrenergic agonists that can be used in the treatment of diseases such as bronchial asthma and chronic bronchitis as a bronchodilator agent in the clinic treatment [1,2,3]. A large amount of CLB can be used as feed additives to significantly promote the growth of the animal and increase the lean rate. Erefore, CLB is known as a lean meat agent [4,5,6]. CLB can be used to increase the lean rate by using a large dose, which is usually more than 10 times that of human medicine. Long-term ingesting CLB from animal foods can cause abnormal physiological reactions such as dizziness, vomiting, muscle tremors, and even death [8]. Illegal and uncontrolled use of CLB can increase the environmental burden and cause environmental pollution [9]
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