Preparation of Magnetic Dummy Template Molecularly Imprinted Polymers for the Determination of Aminoglycosides Antibiotics in Milk

Abstract

In this paper, the efficient magnetic dummy molecular imprinted polymers were firstly synthesized by dummy template (raffinose). The dummy molecularly imprinted layer was polymerized on the surface of Fe3O4 nanoparticles modified with NH2 group. The micromorphology and chemical composites of prepared polymers were detailed characterized. Their adsorption and desorption conditions including extraction time, adsorbent dosage, solution pH, washing solvent, and elution solvent were carefully evaluated. The results showed that the obtained magnetic dummy template molecularly imprinted polymers showed uniform sizes, high magnetic responsivity, core–shell structure, and selectively recognition capacity towards five aminoglycoside antibiotics. Under optimum conditions, magnetic solid-phase extraction coupled high performance liquid chromatography-tandem mass spectrometry for enriching and determining aminoglycoside antibiotics in milk samples was developed. This method showed good linearity (50–1000 μg/kg) with r2 larger than 0.9923. The limits of detection (S/N = 3) were from 3.6 to 9.6 μg/kg. With spiked at three different concentration levels, the recoveries fell into 82.6–114.1% with relative standard deviation from 2.8 to 13.2%. The established method based on magnetic dummy molecular imprinted polymers proved to be highly sensitive and repeatable, providing the promising adsorbent for aminoglycoside antibiotics analysis in the complex samples.