Preparation of IR780 and vincristine-loaded poly (lactic-co-glycolic acid) nanoparticles and antinep-hroblastoma cells proliferation in vitro

Abstract

Objective To prepare IR780 and vincristine (VCR)-loaded poly(lactic-co-glycolic acid)(PLGA) nanoparticles (IR780-VPN) and verify their specific targeting ability and anti-proliferative efficiency in vitro. Methods IR780-VPN were prepared to detect their basic physical and chemical characteristics. SK-NEP1 cells were used as a tumor cell model to verify the specific targeting ability and the anti-proliferative efficiency of IR780-VPN. SK-NEP1 cells were divided into six groups: group A: PBS; group B: free VCR; group C: PLGA nanoparticles; group D: VCR-PLGA nanoparticles; group E: IR780-PLGA nanoparticles; group F: IR780-VPN. Results The average particle size of IR780-VPN was (290.82±3.22) nm, the particle dispersion index (PDI) was 0.024, and the average zeta potential of IR780-VPN was (1.16±0.87) mV. IR780-VPN showed stable physical and chemical properties, and they were spherical, uniform in size, and regular in shape, without aggregation oradhesion under a microscope. The morphology of IR780-VPN was spherical or quasi-spherical with a smooth surface, and the particle size distribution was uniform. The average encapsulation efficiency of IR780-VCR-PLGA nanoparticles was about 72.80%, the average loading efficiency was 2.80%, and the specific targeting efficiency was 91.30%. Many IR780-VPN were observed to target tumor cells in vitro. The cytotoxicity was the highest in group F when compared with group B and group D at the same concentration (P 0.05). The rate of reduced cell proliferation in group A was (2.83±0.32)%. Conclusion IR780-VPN with stable properties have been successfully prepared, which can specifically target tumor cells and have improved anti-proliferative efficiency in vitro. Key words: Targeted nanoparticles; Ultrasound contrast agents; Wilms tumor; Cell proliferation