Preparation of immobilized swainsonine analogs on solid support

Abstract

Golgi α-mannosidase II and lysosomal mannosidase are examples of biologically important α-mannosidases that are difficult to purify. Swainsonine is an effective inhibitor of these α-mannosidases. In this report, analogs of swainsonine bearing an aminoalkyl group at C3 or C6 are synthesized and coupled with Affi-Gel 10 to prepare affinity matrices that may be useful for the preparation of α-mannosidases. Model ligands, where the aminoalkyl group is capped with an acyl group, are evaluated against a commercial α-mannosidase.