Abstract
An immobilized β-glucuronidase enzyme reactor (IMER) based on organic-silica gel hybridized capillary column was prepared by the sol-gel method with acrylamide (AAM) as the organic functional monomer. The effects of the amounts of tetramethyl orthosilicate (TMOS), 3-glycidoxypropyltrimethoxysilane (γ-MAPS), acrylamide (AAM), polyethylene glycol (PEG), and the water bath temperature on the quality of the microscopic structure were studied. After optimization,the monolithic column with good permeability and high mechanical strength was obtained. Then β-glucuronidase was covalently immobilized, and the hydrolysis efficiency of the β-glucuronidase IMER was investigated. As the substrate, O-glucuronides of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL-O-Gluc) were hydrolyzed at room temperature, indicating the high hydrolysis efficiency of the β-glucuronidase IMER, which demonstrated its high potential for the high throughput analysis of tobacco metabolites in the future.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
