Preparation of immobilized enzyme surfaces by radiation technique

Abstract

Immobilized cellulase surfaces were prepared by a simple radiation technique, coating 2-hydroxyethyl methacrylate and tetraethyleneglycol diacrylate on the inner surface of a polyethylene tube. The durability of the surfaces was examined by repeated batch enzyme reactions as a function of monomer and enzyme concentration. The amount of the enzymes located on the surface was about 0.5 mg/cm 2, in which the enzymes did not leak from the polymer matrix. The optimum monomer concentration in the immobilization was 60–70%.