Abstract
Human serum albumin (HSA), a versatile protein carrier for drug delivery, is an ideal material to fabricate nanoparticles for drug delivery systems. These nanoparticles can accumulate in tumor interstitium due to the enhanced permeability and retention effect. The most important characteristics of HSA nanoparticles are particle size, shape, and zeta potential. A chemometric approach was applied for HSA nanoparticles’ size optimization in this study. The effects of three experimental parameters; pressure (P) or power, organic solvent volume (V), and time (T), were investigated under sonication and high-pressure homogenization, using multivariate analysis. The trials were performed based on the Box–Behnken experimental design. The criteria for the appraisal of the descriptive ability of a multinomial were R2 = 0.819, standard error = 20.420, and F-ratio = 19.550. The method was optimized with respect to the nanoparticles’ size as a response. The Box–Behnken experimental design was applied to optimize and trial the robustness of the HSA nanoparticle preparation method.
Highlights
The non-specific distribution of cytotoxic drug molecules and adverse effects are the major drawbacks of conventional chemotherapy methods
The effect of three factors on the average diameter of Human serum albumin (HSA) nanoparticles was evaluated for three times
Acetonitrile is a water-miscible solvent, the results showed that predictable particle size can be achieved for HSA nanoparticles
Summary
The non-specific distribution of cytotoxic drug molecules and adverse effects are the major drawbacks of conventional chemotherapy methods. Nanoparticulate drug delivery systems have been extensively investigated as a robust strategy in targeted drug delivery in the field of pharmaceuticals [1,2,3]. Human serum albumin (HSA) has been shown to be biodegradable, biocompatible, and nonimmunogenic, and it has been used extensively as a protein. Nanotechnology Research Centre, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran SPARC receptors on the surface of cells may facilitate cellular uptake of HSA and release drug conjugates [14, 17, 18]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
