Abstract
In suspension polymerization system, poly(glycidyl methacrylate) (PGMA) microspheres are firstly prepared. Then, ring opening reaction takes place when heparin is bonded on PGMA microspheres to obtain functional PGMA-heparin microspheres. The chemical structure and physicochemical characters of PGMA-heparin microspheres are fully characterized with infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and zeta potential determination. The adsorption properties of PGMA-heparin for lysozyme are mainly investigated. The effects of the main factors on the adsorption properties are explored, furthermore the adsorption mechanism is analyzed in depth, and the adsorption thermodynamics is also researched. The experimental results show that the strong electrostatic interaction is formed between high density of negative charge coming from the carboxyl groups or sulfonic acid groups of heparin and the positive charge of basic protein lysozyme. The adsorption of PGMA-heparin for lysozyme is dependent on the pH value of the medium, and the saturated adsorption amount of PGMA-heparin for lysozyme possesses a maximum at pH 8, which reaches up to 654 mg/g. The adsorption of PGMA-heparin for lysozyme is an exothermic process which is driven by entropy, and the adsorption amount decreases with increasing the temperature. Open image in new window
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