Abstract
Fumed silica (FS) is one of the few nanomaterials used in large scale in the production of several industrial products. In addition, the very low toxicity of these nano-sized particles led them to become excellent raw materials to develop novel applications in the field of downstream processing of bioproducts. In this work, we prepared and characterized a colloidal composite based on fumed silica with protein adsorption properties. This nanocomposite was prepared by radiation-induced polymerization of glycidyl methacrylate (GMA) dissolved in an aqueous suspension of FS without stirring. According to the initial GMA/FS ratio, mono-disperse nanoparticles from 60 to 195nm were obtained. Sulfonic nanoparticles allowed reversible lysozyme adsorption up to 270mg/g. The nanomaterials prepared have potential application as protein-capture materials in the field of downstream processing of proteins.
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