Preparation of Fucoidan-Based Electrospun Nanofibers and Their Interaction With Endothelial Cells.

Yiwen Chen,Zhanyi Sun,Huilin Zhu,Peili Shen,Yuanping Hao,Qihui Zhou

doi:10.3389/fbioe.2021.739209

Abstract

Sulfated polysaccharide fucoidan (FD) is widely applied in biomedical applications owing to its outstanding bioactivities. In addition to the biochemical features, the architecture of biomaterials plays a critical role in tissue repair and regeneration. Particularly, nanofibers have elicited great interest due to their extracellular matrix-like structure, high specific surface area, and favorable biological properties. Herein, chitosan-modified FD/ultra-high molecular weight polyethylene oxide (UHMWPEO) nanofibers are developed via green electrospinning and electrostatic interaction for studying their interaction with endothelial cells. The appropriate solvent is screened to dissolve FD. The electrospinnability of FD/UHMWPEO aqueous solutions is greatly dependent on the weight ratios of FD/UHMWPEO. The incorporation of UHMWPEO significantly improves the electrospinnability of solution and thermo-stability of nanofibers. Also, it is found that there is good miscibility or no phase separation in FD/UHMWPEO solutions. In vitro biological experiments show that the chitosan-modified FD/UHMWPEO nanofibers greatly facilitate the adhesion of endothelial cells and inhibit the attachment of monocytes. Thus, the designed FD-based nanofibers are promising bio-scaffolds in building tissue-engineered blood vessels.

Highlights

In the past few decades, marine polysaccharides have gained increasing attention in the area of diversified biomedical applications owing to their inherentphysicochemical features, such as biocompatibility, biodegradability, favorable bioactive, biomechanical properties, and structural functionalities (Bidarra et al, 2014; Fernando et al, 2019; Hao et al, 2020; Jana et al, 2020; Yin et al, 2021; Zheng et al, 2021)
It was reported that other nature polymers [e.g., chitosan (CS), cellulose, sodium alginate, protein] with a small amount of ultra-high molecular weight polymer (UHMWP) [e.g., polyethylene oxide (PEO), polyvinyl alcohol (PVA), polyvinyl pyrrolidone (PVP)] can allow their preparation in nanofibers via electrospinning (Zhang et al, 2008; Li et al, 2015)
It was demonstrated that the selection of solvent is critical to determine material solubility, viscoelasticity, electrical conductivity and electrospinnability of the solution, as well as the productivity and morphology of nanofibers (Zhou et al, 2013; Casasola et al, 2014)

Summary

Introduction

In the past few decades, marine polysaccharides have gained increasing attention in the area of diversified biomedical applications owing to their inherent (bio)physicochemical features, such as biocompatibility, biodegradability, favorable bioactive, biomechanical properties, and structural functionalities (Bidarra et al, 2014; Fernando et al, 2019; Hao et al, 2020; Jana et al, 2020; Yin et al, 2021; Zheng et al, 2021). It was reported that other nature polymers [e.g., chitosan (CS), cellulose, sodium alginate, protein] with a small amount of ultra-high molecular weight polymer (UHMWP) [e.g., polyethylene oxide (PEO), polyvinyl alcohol (PVA), polyvinyl pyrrolidone (PVP)] can allow their preparation in nanofibers via electrospinning (Zhang et al, 2008; Li et al, 2015). In this sense, the combination of FD and UHMWP could be considered to address the issue of spinnability

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Conclusion