Abstract
An inexpensive and dependable method for freeze-drying biological tissue for transmission electron microscopy has been developed. Reproducible results can be attained if the following procedure is carefully followed.1) The primary components for the freeze-drier are shown assembled in figure 1. Preheat the 1000 ml pumping chamber containing 5 Å molecular sieve for at least 12 hours at 150° C. Connect the isolation valve to the chamber, close the valve, and stopper the flask with a #8 Neoprene stopper. Supporting the chamber with a ring stand clamp, place it in a Dewar filled with liquid nitrogen and allow it to equilibrate. Connect the drying chamber to the isolation valve and set it up in another Dewar without liquid nitrogen. Remove the #7 Neoprene stopper with its thermometer and specimen block and suspend it apart from the drying chamber with a ring stand clamp. The bulb of the thermometer is not tightly positioned in the specimen block at this time but is about ” above it.
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