Abstract
In this work, a novel molecularly imprinted polymer (MIP) with water-soluble CdTe quantum dots (QDs) was synthesized by oil-in-water Pickering emulsion polymerization using whole Listeria monocytogenes as the template. Listeria monocytogenes was first treated by acryloyl-functionalized chitosan with QDs to form a bacteria–chitosan network as the water phase. This was then stabilized in an oil-in-water emulsion comprising a cross-linker, monomer, and initiator, causing recognition sites on the surface of microspheres embedded with CdTe QDs. The resulting MIP microspheres enabled selective capture of the target bacteria via recognition cavities. The target bacteria Listeria monocytogenes was detected. Scanning electron microscopy (SEM) characterization showed that the MIPs had a rough spherical shape. There was visual fluorescence detection via quenching in the presence of the target molecule, which offered qualitative detection of Listeria monocytogenes in milk and pork samples. The developed method simplified the analysis process and did not require any sample pretreatment. In addition, the fluorescence sensor provided an effective, fast, and convenient method for Listeria monocytogenes detection in food samples.
Highlights
Foodborne pathogens are an emerging global public health problem [1]
There was no significant difference between the Fourier transform infrared (FT-IR) spectra of the Molecularly imprinted polymer (MIP) and non-imprinted polymer (NIP), the template was removed completely
The results indicate that the high recognition of thethe imprinted polymer sites thatbacteria
Summary
Foodborne pathogens are an emerging global public health problem [1]. Even with increased awareness in food safety and quality control, there are still about 48 million cases of foodborne diseases in the United States every year [2,3]. Salmonella, Escherichia coli O157:H7, Staphylococcus aureus, Listeria monocytogenes, and Bacillus cereus are the most common foodborne pathogens [4,5]. Listeria monocytogenes (L. monocytogenes) is more likely to cause death and is associated with listeriosis. Aside from its high fatality rate (20–30%), listeriosis can cause many diseases such as sepsis and meningitis. It readily infects immunocompromised people and in particular neonates, pregnant women, and people over age 65 [6,7]. Fruits, vegetables, seafood, milk, and dairy products are common foods associated with Listeria monocytogenes [8,9]
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