Abstract
The fluorocarbon purification technic was applied to purification of complement-fixing antigens prepared in monkey kidney cultures from ECHO prototype strains. The effect of serum in tissue culture medium on the effect of fluorocarbon was studied. Adding serum to synthetic tissue culture medium improved the titers of ECHO CF antigens. Lactalbumin hydrolysate media containing serum represented the best media of several tried for the preparation of ECHO CF antigens in monkey kidney culture. The untreated antigens, when tested with guinea pig sera prepared by immunizing animals with monkey kidney grown virus, gave strong monkey kidney tissue reactions. However, fluorocarbon treatment removed the nonspecific tissue reaction and “purified” antigens gave specific reactions in CF tests with ECHO antisera prepared in guinea pigs and in monkeys.
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