Preparation of dual-modality yttrium-90 radiolabeled nanoparticles for therapeutic investigation

Abstract

Abstract Magnetic Fe3O4 nanoparticles with narrow size distribution were synthesized by simple and high yielded co-precipitation technique using ferrous salts with a molar ratio of Fe3+/Fe2+=2. After coating of the nanoparticles with Stöber silica (SiO2@Fe3O4), nanoparticles were functionalized by amine groups. Then chelator molecules diethylenetriaminepentaacetic (DTPA) and tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) were coupled to the APTS-SiO2@Fe3O4 to chelating Y-90 radiometal that makes these nanoparticles a suitable agent for therapeutic application as dual-modality PET/MRI imaging. The results show the coupling of DTPA takes place better than DOTA. Synthesis of magnetic nanoparticles (MNPs) was followed by structure identification using XRD, SEM, TGA and IR techniques. In order to trace MNPs biodistribution, the radiolabeled MNPs-DTPA were prepared using 90Y (production of 90Y/90Sr generator) with a good labeling efficiency (about 92%, RTLS method). The biodistribution of the radiolabeled MNPs was checked in normal male rats up to 24 h compared to free Y3+. The data shows that the tracer accumulation is in reticuloendothelial tissue while the stability of the complex is highly retained.