Abstract
The rapid purification of biomaterials such as DNA, RNA, and antibodies has attracted extensive attention, and research interest has increased further with the COVID-19 pandemic. In particular, core–shell-structured superparamagnetic nanoparticles have been continuously studied for their application as biopurification materials. It has been reported that Fe3O4@SiO2 nanoparticles are one of the most promising candidates for separating nucleic acids via a simple and rapid process. This study proposed a fabrication method for dual-layered Fe3O4@SiO2 nanoparticles, in which the density of the SiO2 shell was controlled using an intermediate surfactant during the SiO2 coating. After the fabrication of dual-layered Fe3O4@SiO2 nanoparticles, structural, morphological, and magnetic analyses were conducted. The results showed that the Fe3O4 nanoparticles were surrounded by a dense layer 15.6~27.9 nm thick and a porous layer 24.2~44.4 nm thick, and had superparamagnetic properties with high saturated magnetization at room temperature (86.9 emu/g). Then, the optimal conditions for the biopurification material were suggested based on analysis of the selective separation of plasmid DNA.
Highlights
Superparamagnetic nanoparticles are considered promising materials in various fields owing to their magnetic properties [1,2,3,4,5,6,7]
Since the core–shell structure could take advantage of both an Fe3 O4 core, which could control nanoparticles through its superparamagnetic properties, and an SiO2 shell, which can be used for purifying biomaterials such as nucleic acid and antibodies, surface functionalization through silane grafting, and enhancing chemical and thermal stabilities, core–shell-structured Fe3 O4 @SiO2 nanoparticles have received extensive attention, and a large number of studies have been conducted on the fabrication method and application of
We proposed a preparation method based on the core–shell structure for dual-layered Fe3 O4 @SiO2
Summary
Superparamagnetic nanoparticles are considered promising materials in various fields owing to their magnetic properties [1,2,3,4,5,6,7]. To purify biomaterials using superparamagnetic nanoparticles, a surface functionalization process is necessary, and many related studies have been reported based on both basic research and practical applications [6,8,9]. Since the core–shell structure could take advantage of both an Fe3 O4 core, which could control nanoparticles through its superparamagnetic properties, and an SiO2 shell, which can be used for purifying biomaterials such as nucleic acid and antibodies, surface functionalization through silane grafting, and enhancing chemical and thermal stabilities, core–shell-structured Fe3 O4 @SiO2 nanoparticles have received extensive attention, and a large number of studies have been conducted on the fabrication method and application of. We discuss the effects of the types of shell structures on the dispersion and magnetic properties of Fe3 O4 @SiO2 nanoparticles, which could further affect the plasmid DNA purification
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