Preparation of deuterated abscisic acid metabolites for use in mass spectrometry and feeding studies

Abstract

Four deuterium-labelled ABA catabolites of high isotopic purity were prepared for hormone profiling studies using HPLC-ESI-MS-MS. The catabolites, namely [7′,7′,7′-d3]-PA, [7′,7′,7′-d3]-DPA, [5,8′,8′,8′-d4]-7′-hydroxyABA and [4,5,8′,8′,8′-d5]-ABA glucose ester were prepared either by biotransformation or chemical synthesis starting with labelled ABA. The labelled compounds are employed as internal standards for plant hormone profiling by liquid chromatography–electrospray–tandem mass spectrometry. Copyright © 2005 John Wiley & Sons, Ltd.