Preparation of crude transfer RNA and chromatographic purification of five transfer RNAs from calf liver

Abstract

Five tRNAs (aspartic acid-2, leucine-1, lysine-2, phenylalanine, and serine-3) were isolated from crude calf liver tRNA by chromatographic procedures involving benzoylated DEAE-cellulose, reversed-phase chromatography, and DEAE-Sephadex A-50. Calculated activities (amino acid acceptance per 3′-terminal adenosine) were from 87 to 102 %. Crude calf liver tRNA contains much smaller quantities of RNA contaminants than crude tRNA from the mature beeves. The procedures for the preparation of the crude tRNA and chromatographic steps for isolation of these five tRNAs are amenable to scale-up for the preparation of larger samples.