Preparation of cross-linked silk fibroin film by γ-irradiation and their application as supports for human cell culture

Abstract

This study described about preparation of the cross-linked silk fibroin (SF) film by γ-irradiation of the casted SF film, which is fabricated from aqueous solution regenerated via fibers of cocoons and their application as supports for human cell culture. The properties of cross-linked SF film were evaluated by FTIR spectroscopy, contact angle, solubility to water, thermal analysis, surface area analyzer, and morphology via scanning electron microscopy (SEM). The cross-linked SF films were not dissolved in water and exhibited the rough surface morphology, large surface area, and good thermal properties. The human fibroblast cell (CCD-986sk) and embryo kidney-ft cell were well growed on the surface of cross-linked SF film supports prepared by γ-irradiation. The cross-linked SF film prepared by γ-irradiation can be used as biomaterials for human cell culture.