Abstract

Details are given for the preparation of low molecular weight copolymers of isobutyl methacrylate and styrene for use in mounting microscopical sections between the slide and cover glass. Any refractive index in the range from 1.477 to 1.590 is available, depending upon the proportions of monomers used. A refractive index of 1.550 is recommended for general purposes, though other indices might be desirable in special cases including phase microscopy. The resin gives solutions in toluene of suitable fluidity at usable concentrations, adheres satisfactorily to glass, and has excellent light-transmission. The features which make it especially valuable are its optimum refractive index and its permanent water-whiteness.Copolymers of isobutyl methacrylate and styrene may also be used to prepare plastic mounts of thick sections or gross specimens, which are optically cleared by the plastic mountant.

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