Preparation of coaxial electrospinning nanofibers for vascular endothelial growth factor delivery

Abstract

Objective To construct coaxial electrospinning nanofibers with poly (e-caprolaetone)(PCL) as the shell and BSA and vascular endothelial growth factor (VEGF) as the core,expore its ability for VEGF delivery in vitro,and examine the biological activity of VEGF released.Methods The morphology of VEGF coaxial electrospinning nanofibers was observed at the voltage from 15-18 kV.At 1st,3rd,7th,15th,and 30th day,bovine serum albumin (BSA)/PCL was 17％,34％,38％,45％ and 56％,BSA/PCL-polyethyleneglycol (PEG) was 30％,45％,80％,89％ and 92％,and VEGF release was 5％,10％,20％,60％,90％,respectively.The curve of time-cumulative release percentage was drawn.At last,the biological activity of VEGF released was tested.Results The diameters of nanofibers at the voltage of 15,16,17 and 18 kV were (282.00±43.57),(199.13 ±32.87),(182.00±27.74),(159.00 ±36.33) nm respectively.The VEGF was released gradually within 30 days,and the cumulative release percentage was almost 98％.The biological activity of the released VEGF has no significant difference from the fresh VEGF (P ＞ 0.05),but significant difference from no-VEGF group (P ＜ 0.05).Conclusion We construct the VEGF coaxial electrospinning nanofibers with PCL as the shell and BSA and VEGF as the core,and can change the diameter of nanofibers by adjusting the voltage.The nanofobers can retain biological activity of VEGF and release VEGF gradually,and can also adjust the releasing by adding PEG. Key words: Drug release; Vascular endothelial growth factor; Nanofiber